ATAC-seq

Laiumiunix · 04-03-2018, 03:35 PM

HI,

I'm a little confused how to orders the primers too:
Just to be sure if I understand:

For each sample:

I will need:

Sample1
Ad1_noMX:
Ad2.1_index

Sample2
Ad1_noMX:
Ad2.2_index:

etc
In terms to order the primers if I follow the list from (Jason D Buenrostro paper):

I should be order:

Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG

Basically the second primer I have to put the index sequence to order:
index+sequence?
Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT

Thanks

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04-03-2018, 03:35 PM	#1
Laiumiunix Junior Member Location: California Join Date: Apr 2018 Posts: 4	ATAC-seq HI, I'm a little confused how to orders the primers too: Just to be sure if I understand: For each sample: I will need: Sample1 Ad1_noMX: Ad2.1_index Sample2 Ad1_noMX: Ad2.2_index: etc In terms to order the primers if I follow the list from (Jason D Buenrostro paper): I should be order: Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG Basically the second primer I have to put the index sequence to order: index+sequence? Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT Thanks