Hi everyone,
I am request to know the adapter and primer sequence information of the following kits:
1) Truseq PE cluster Kit V3 cBot-Hs
2) Truseq Rapid PE cluster Kit-Hs
3) Nextera XT DNA preparation kit
4) Truseq RNA sample preparation kit.
The reason I am asked those sequences is that I am pretty confused about the read 2 primer. As in old GA protocol, we often use PE-read 2 primer. But, there is another read 2 primer called multiplexing read 2. Both primer only 13nt same at the end which has a pretty low TM~ 42.
And I am comfortable to use SMART strand switch to make RNA-seq library ourself. However, I am not sure which adapter sequence I have to use this time. PE-read 2 or Multiplexing read 2.
Thank you very much.
I am request to know the adapter and primer sequence information of the following kits:
1) Truseq PE cluster Kit V3 cBot-Hs
2) Truseq Rapid PE cluster Kit-Hs
3) Nextera XT DNA preparation kit
4) Truseq RNA sample preparation kit.
The reason I am asked those sequences is that I am pretty confused about the read 2 primer. As in old GA protocol, we often use PE-read 2 primer. But, there is another read 2 primer called multiplexing read 2. Both primer only 13nt same at the end which has a pretty low TM~ 42.
And I am comfortable to use SMART strand switch to make RNA-seq library ourself. However, I am not sure which adapter sequence I have to use this time. PE-read 2 or Multiplexing read 2.
Thank you very much.