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Yes! 100 ng! Sorry
I corrected the post. And I put all the reaction in the gel, so it should be 100 ng too.
I corrected the post. And I put all the reaction in the gel, so it should be 100 ng too.
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@Castrolsc The digestion profile will vary by sample type, but it looks like the digestion for your Sperm DNA worked fine. There is material in the range you need for library generation, and the expected microsatellite bands are visible. Why do you think it isn't working? Have these samples failed when used for library prep?Josh KinmanComment
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I continued with the protocol and my Ct values after qPCR were very right (around 19-20), according to the company we should expect something around 10-17, then we started to investigate each step before that and put the digestion product in the gel to see how it looks like. I know that RRBS will only get around 10% of whole genome, but my doubt now is if I could prepare a library with this few amount of bands around 100 to 500bp, because most part of my DNA is >2000bp.Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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