Hello,
Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol?
We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if we eluted in perhaps half the volume, we might get higher concentrations for running on the sequencers.
It would mean that the test step prior to beginning a run would technically be a fail as it tests volumes in both the loading and elution wells. Do you think that would be a big issue?
Thanks in advance for your thoughts!
Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol?
We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if we eluted in perhaps half the volume, we might get higher concentrations for running on the sequencers.
It would mean that the test step prior to beginning a run would technically be a fail as it tests volumes in both the loading and elution wells. Do you think that would be a big issue?
Thanks in advance for your thoughts!
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