How to detect heterozygous (double) peaks from a Sanger sequence?
I found an article (http://seqcore.brcf.med.umich.edu/do...interpret.html) suggest Sequencher, Mutation Surveyor, and polyphred can detect heterozygous bases. But I haven't had luck in applying any of them to approach my purpose.
Any one has experiences in using any software for detecting heterozygous bases from a Sanger sequence?
I found an article (http://seqcore.brcf.med.umich.edu/do...interpret.html) suggest Sequencher, Mutation Surveyor, and polyphred can detect heterozygous bases. But I haven't had luck in applying any of them to approach my purpose.
Any one has experiences in using any software for detecting heterozygous bases from a Sanger sequence?
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