Hi there,
I'm a newbie in bioinformatics and I encounter a simple problem with FLASH (Fast Length Adjustment for SHort reads).
I'm working on Mac OS X and followed the instructions to download, untar the tarball and compile using $make:
$ make
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o flash.o flash.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o combine_reads.o combine_reads.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o fastq.o fastq.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o util.o util.c
cc -pthread flash.o combine_reads.o fastq.o util.o -lz -o flash
clang: warning: argument unused during compilation: '-pthread'
But when I ran the command I got an error message:
$ flash -o flashout_trimmed --interleaved-output trimmedR1.fastq trimmedR2.fastq
-bash: flash: command not found
Can anyone help with that?
Thank you very much in advance!
I'm a newbie in bioinformatics and I encounter a simple problem with FLASH (Fast Length Adjustment for SHort reads).
I'm working on Mac OS X and followed the instructions to download, untar the tarball and compile using $make:
$ make
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o flash.o flash.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o combine_reads.o combine_reads.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o fastq.o fastq.c
cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o util.o util.c
cc -pthread flash.o combine_reads.o fastq.o util.o -lz -o flash
clang: warning: argument unused during compilation: '-pthread'
But when I ran the command I got an error message:
$ flash -o flashout_trimmed --interleaved-output trimmedR1.fastq trimmedR2.fastq
-bash: flash: command not found
Can anyone help with that?
Thank you very much in advance!
Comment