Hello,
I am planning to run a HiSeq 2500 rapid run with a different number of cycles for reads 1 and 2 (e.g. 250 x 50). I know that this layout produces a lot worse quality than 150 x 150, however what I need more in my current project is longer and more reads in spite of poor quality reads (I'm happy with phred score 20).
Does anyone have an experience of this kind of runs?
Thank you
I am planning to run a HiSeq 2500 rapid run with a different number of cycles for reads 1 and 2 (e.g. 250 x 50). I know that this layout produces a lot worse quality than 150 x 150, however what I need more in my current project is longer and more reads in spite of poor quality reads (I'm happy with phred score 20).
Does anyone have an experience of this kind of runs?
Thank you
Comment