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Tools for identifying/verifying protein (without accession number)
If I have a series of protein sequences (experimentally obtained) that yield no results from BLAST, are there any other online/software tools that can be used to either identify or verify that these proteins exist?
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If you obtained these sequences experimentally then they definitely exist
Have you tried psi-blast or better yet delta-blast @NCBI? -
They're not annotated in databases such as UniProt/Swiss-Prot so I'm not sure how I can "prove" that they are indeed real and transcribed from genes.
All I have are the protein sequences themselves though, won't I need accession numbers or ID to run them through psi/delta-blast?Comment
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You just need fasta sequence for doing delta- or psi-blast. You can limit your search against swissprot or refseq if you want well annotated proteins.Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
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