Hi all,
I would like to run an RNA differential expression analysis on two groups (two cancer subtypes) but my number of samples in the groups is not even close to equal.
I have one group with about 120 samples and the other one has around 25 samples, is it O.K to run DEseq or EdgeR on such different size groups ? Would the possible bias be corrected somehow ? If not, What is the maximal group difference I can work with in DE?
Thanks,
Best,
Moriah
I would like to run an RNA differential expression analysis on two groups (two cancer subtypes) but my number of samples in the groups is not even close to equal.
I have one group with about 120 samples and the other one has around 25 samples, is it O.K to run DEseq or EdgeR on such different size groups ? Would the possible bias be corrected somehow ? If not, What is the maximal group difference I can work with in DE?
Thanks,
Best,
Moriah
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