Originally posted by arvid
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Originally posted by billstevens View PostThanks for the advice! I've been looking for a machine that has 8 core, but I couldn't find anything that was commercially available for that (unless it was a MacPro, which was $12000). What kind of PC do you have? Did you just build it yourself?
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Originally posted by alexdobin View Post1. Yes, STAR detects insertions and deletions
2. The minimum intron size is determined by the --scoreDelLmax (=20 by default). If the genomic gap is below that value, it's considered deletion, otherwise - intron. The maximum intron size is approximately determined by winAnchorDistNbins*2^winBinNbits = 9*2^16~600kbases by default, which we think works well for mammalian genomes, but you can increase it at will by increasing --winAnchorDistNbins. There is yet another parameter --winFlankNbins that determines maximum gap for lower confidence introns: winFlankNbins*2^winBinNbits.
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Originally posted by arvid View PostOk, I might try it out. I suppose you can *lower* these settings as well? For e.g. Arabidopsis I'd set something like 3-10k bases for max intron size (3 for lower FDR, 10 for maximum sensitivity) and ~10 bases as minimum intron size... At the moment GSNAP performs better than TopHat with such settings (in my hands at least) - but I'd be interested in trying alternatives.
--scoreDelLmax 10 --winAnchorDistNbins 3 --winBinNbits 12 --winFlankNbins 1
That would give the max intron size for high/low confidence of 12/4kb.
If you decide to try STAR and have any questions/problems please email me at dobin~at~cshl.edu
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