Hi,
We have just started to make our own TruSeq libraries and do the Truseq multiplex exome capture. We found that the second enrichment did not have any effect on the input (i.e. the amount we put in was the amount we got out after the second enrichment reaction).
Can some one please comment on their experience with TruSeq exome capture.
1. If you put in 500ng (x 6 samples = 300ng) of the pooled TruSeq library for Truseq multiplex exome capture, what is the yield after the first capture?
2. What is the yield after the second capture?
3. How much of the capture material do you need for sequencing on a hiseq?
thanks
Christoph
We have just started to make our own TruSeq libraries and do the Truseq multiplex exome capture. We found that the second enrichment did not have any effect on the input (i.e. the amount we put in was the amount we got out after the second enrichment reaction).
Can some one please comment on their experience with TruSeq exome capture.
1. If you put in 500ng (x 6 samples = 300ng) of the pooled TruSeq library for Truseq multiplex exome capture, what is the yield after the first capture?
2. What is the yield after the second capture?
3. How much of the capture material do you need for sequencing on a hiseq?
thanks
Christoph
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