Hi,
We are demultiplexing some in house barcodes, 5 nt in length as mentioned in a previous post for 2 x 150 Miseq runs
When this is done, there is a very high frequency of mismatch between R1 and R2 in barcode splitter. The barcode file used is the same. Is there any need to use the complment, or the reverse for the splitting of R2?
Thanks a lot
We are demultiplexing some in house barcodes, 5 nt in length as mentioned in a previous post for 2 x 150 Miseq runs
When this is done, there is a very high frequency of mismatch between R1 and R2 in barcode splitter. The barcode file used is the same. Is there any need to use the complment, or the reverse for the splitting of R2?
Thanks a lot
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