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  • quality of RNA needed for prokaryotic RNA-seq?

    Hi, I'm new to RNA-Seq and am looking for some information on the quality/size profile of RNA needed to produce high-quality data on either the 454 or Illumina platform. We are working with some environmental samples, and the goal is to sequence total prokaryotic community RNA. A MoBio soil RNA extraction kit produced decent results from an E.coli test culture but a freshly collected environmental sample looks rather degraded to my eye (see attached Bioanalyzer trace from the RNA Pico kit). I haven't found anything information on how to connect Bioanalyzer trace quality with library outcomes. Can anyone help?
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    Hey, did you ever end up finding out what degree the quality control measures correlate with the sequenced library characteristics? Would be real useful info, thanks.

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