We did a 2x300 run for a TCR library recently. Passed spec (77.5% Q30 @ 939k with 15% PhiX), but clear tailing towards the ends of both R1 and R2 as well.
I could see error rates approaching 5% being problematic for many if not most applications.
We'll be sticking with 2x250 or 2x150 chemistry for the time being, unless specifically requested by a client.
I could see error rates approaching 5% being problematic for many if not most applications.
We'll be sticking with 2x250 or 2x150 chemistry for the time being, unless specifically requested by a client.
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