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  • help! what is a paired-end read?

    I am a newer in this area.
    Could anybody tell me what is a paired-end read?

    someone told me that a paired-end read is a read with the two 3' end sequences were sequenced.

    for example: a fragment of DNA strands like this

    3' 5'
    ATTTGCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATTCGG
    5' 3'

    and this is used in an assembly process when sequencing.

    I am confused by his answer.
    Is his answer right? if not, please give me a right answer!
    Thank you N*very much!

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    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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    Senthil Palanisami

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