Good day all,
I am new to analyzing RNA-seq data and am trying to understand how cufflinks calls a transcript significant or not between two conditions?
I ask this because when I assess some of the transcripts that are called not significant by cufflinks with an ANOVA or Kruskal-Wallis test, I get that it is significantly different...
What should I do in a scenario like that? Should I go with cufflinks or other statistical tests?
I would appreciate any assistance in this matter..
Thank you
I am new to analyzing RNA-seq data and am trying to understand how cufflinks calls a transcript significant or not between two conditions?
I ask this because when I assess some of the transcripts that are called not significant by cufflinks with an ANOVA or Kruskal-Wallis test, I get that it is significantly different...
What should I do in a scenario like that? Should I go with cufflinks or other statistical tests?
I would appreciate any assistance in this matter..
Thank you
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