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  • Setting up a 454 Bioinformatics center

    We are expanding our Bioinformatics Core to handle 454 data analysis from two dedicated Titanium sequencers. Our users have several applications in mind: lots of metagenomics, some de novo sequencing, some resequencing, some transcription analysis. And I need to plan for having both sequencers running at capacity.

    Two questions:
    1) which are the best Cores that are already doing this sort of thing, and whom do I contact at them for sage advice?
    2) any idea how much horsepower and storage I need? (I realize it's impossible to answer that, but any rules of thumb or words of wisdom would help).
    3) who has the best integrated software for the relatively naiive user? (for example, RDP at MSU has a decent pipeline in development)

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  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

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