Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • sff into fasta/qual files

    Hi everybody!

    I have a problem: when I have seen my fasta/qual files of my 454Jn run, I've realized that these files are 0kb, totally empty!!

    anybody knows what happens?

    thank you!!

    Isabel

  • #2
    Did you solve this problem? How did you generate your .fasta and .qual files?

    Comment


    • #3
      Hi Glovesfox!

      Finally, I couldn't generate the files, so if you have the same problem, I can't say you anything.

      Sorry!

      Isabel

      Comment


      • #4
        You still haven't provided any information on what you did. Has the run successfully finished? What have you done to create which fasta/qual files? What about your SFF files? Are these empty or not?

        I don't have a crystal ball ;-)

        Comment


        • #5
          I don't have the same problem - I am trying to help you!
          You must have done something to generate fasta and qual files from the sff file. I'm guessing you used a tool called sfffile on the command line?

          Comment


          • #6
            Some questions to help us understand so we can help you:

            Are you talking about the .fna and .qual files that are automatically created as part of the process, or are these files you created later from the .sff file?

            Have you looked at the run in the Run Browser? How did that look?

            What is the file size of the .sff file?

            Comment


            • #7
              Sorry!

              The sff file only has 2kb. This run was a totally disaster, so I think that we can forget it.

              Thanks so much, and sorry, I'm totally new at this and I don't understand the most of the thinks.

              Comment

              Latest Articles

              Collapse

              • seqadmin
                Strategies for Sequencing Challenging Samples
                by seqadmin


                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                03-22-2024, 06:39 AM
              • seqadmin
                Techniques and Challenges in Conservation Genomics
                by seqadmin



                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                Avian Conservation
                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                03-08-2024, 10:41 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by seqadmin, 03-27-2024, 06:37 PM
              0 responses
              12 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-27-2024, 06:07 PM
              0 responses
              11 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-22-2024, 10:03 AM
              0 responses
              53 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-21-2024, 07:32 AM
              0 responses
              69 views
              0 likes
              Last Post seqadmin  
              Working...
              X