Hello,
I am considering to use the new NEBNext Microbiome Enrichment kit on human mucosal and fecal samples. There is a difference in % of human DNA between these two samples, so I am not expecting the same % enrichment between these two samples.
Does anyone have experience with this kit? What is the % enrichment you usually get, for what types of samples? Did anyone look at bias -i.e. is there taxonomic bias in the enrichment?
There is a recent PLoS One paper out on the method, but I was wondering whether anyone has any direct experience.
Thanks!
I am considering to use the new NEBNext Microbiome Enrichment kit on human mucosal and fecal samples. There is a difference in % of human DNA between these two samples, so I am not expecting the same % enrichment between these two samples.
Does anyone have experience with this kit? What is the % enrichment you usually get, for what types of samples? Did anyone look at bias -i.e. is there taxonomic bias in the enrichment?
There is a recent PLoS One paper out on the method, but I was wondering whether anyone has any direct experience.
Thanks!
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