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Old 09-04-2014, 09:05 AM   #1
soleulloa
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Location: Chile

Join Date: Aug 2011
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Default Is there relationship between library preparation and low quality data?

Hi all!

If:
- all the paramethers about the initial DNA are OK (quantity, 260/280, 260/230 and integrity)
- Bioanalyzer results are OK
- library post OT2 is OK
- run results are OK (% loading wells, low policlonality, a lot of MB, size reads, etc)

Is possible that the low data quality has relationship with the "library prep" or is just a PGM problem?

(this situation is just in one case, the other data runs are OK)

Thanks in advance!
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