Hi Everyone,
I'm in a situation whereby I need to use two extra adapters than the 6 adapters that are currently shipped with each set of the TruSeq RNA-seq prep kit and at the moment we only have one set. We have both sets of adapters for the TruSeq Genomic DNA prep kit (i.e. all 12 adapters).
I know the Genomic DNA and RNA-seq adapters are the same, but the concentration is different. Has anyone used the adapters from the DNA kit in the RNA sample prep? I think it used to be a 10 fold dilution of adapters using the 'old', non TruSeq kits.
Cheers,
Scott.
I'm in a situation whereby I need to use two extra adapters than the 6 adapters that are currently shipped with each set of the TruSeq RNA-seq prep kit and at the moment we only have one set. We have both sets of adapters for the TruSeq Genomic DNA prep kit (i.e. all 12 adapters).
I know the Genomic DNA and RNA-seq adapters are the same, but the concentration is different. Has anyone used the adapters from the DNA kit in the RNA sample prep? I think it used to be a 10 fold dilution of adapters using the 'old', non TruSeq kits.
Cheers,
Scott.
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