Hi:
I typically pool 6 TruSeq libraries with P5/P7 adapter pairs (2 4 5 6 7 12) per lane, as per Illumina's recommended pooling for balanced indexes.
I need to pool 8 libraries, and am having a difficult time finding the pooling strategy recommended for this number. Does anyone have experience with this strategy? In my notes from Illumina, I only have recommendations for 2, 3, 4, and 6 pooled libraries.
Thanks,
ap
I typically pool 6 TruSeq libraries with P5/P7 adapter pairs (2 4 5 6 7 12) per lane, as per Illumina's recommended pooling for balanced indexes.
I need to pool 8 libraries, and am having a difficult time finding the pooling strategy recommended for this number. Does anyone have experience with this strategy? In my notes from Illumina, I only have recommendations for 2, 3, 4, and 6 pooled libraries.
Thanks,
ap
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