Hi there,
I have three samples which were sequenced on a MiSeq. I have attached the R1 and R2 quality per tile for sample 3 and R2 for samples 1 and 2.
For the reverses I get a quality per tile fail for R2 with almost identical plots for all three samples.
After trimming (which normally works pretty well!!) things seem worse.
Is there something else I should be doing (e.g. re-trimming/removing affected reads)?
Any help/advice would be great!!! Thanks in advance!!!
I have three samples which were sequenced on a MiSeq. I have attached the R1 and R2 quality per tile for sample 3 and R2 for samples 1 and 2.
For the reverses I get a quality per tile fail for R2 with almost identical plots for all three samples.
After trimming (which normally works pretty well!!) things seem worse.
Is there something else I should be doing (e.g. re-trimming/removing affected reads)?
Any help/advice would be great!!! Thanks in advance!!!