I want to do the transcriptome annotiation for wheat, which is a hexapliod plant. Since there has no reference genome sequence now, we are going to sequence using pacbio RNA-seq to get the full length isoform sequences as many as possible. I have four samples and have sequenced using HiSeq 2000, then I am going to do the pacbio RNA-seq. So should I do the sequcing combined all samples together or just sequencing seperately? Is there any influence with my hexaploid wheat data analysis? BTW, should I using the IsoSeq pipline or the PacBioToCA/LSC error correction pipline?
Thanks a lot O(∩_∩)O~
Thanks a lot O(∩_∩)O~
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