Hi All, I'm planning on sequencing a highly repetitive vertebrate genome (~1.6 GB genome, ~42% repetitive) using mostly Illumina reads (50X short insert and 50X 3kb insert, PE 150bpx2 reads). I was looking into PacBio reads-- it would cost about $4k to do the genome at 1x, assuming ~200MB of good reads per SMRTcell. I know that it's recommended to have 10-15X PacBio coverage for gap filling and scaffolding, but the cost is a bit too high for me to do that. So, would it even be worth it to get a PacBio run done with 1X coverage? Thanks for any thoughts,
jwag
jwag
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