hi all,
I know that there have been many posts about readgroup in SAM/BAM format. But I am still confused.
Assume I have a sample named "Treated"sequenced with Illumina HiSeq platform. There are 5 pairs of the fastq files. I map each of the small fastq file using BWA and add read group when I merge the pairs of small bam files. For the first pair of bam files, can I just use "Treated_1" as its readgroup and "Treated_2" for the 2nd pair of bam files?
An opposite question: if I merge the fastq files for one end to get a file s1.fastq, then I merge the fastq files for the other end to get s2.fastq. Then I removed the original 5 pairs of original small fastq files. My question is, is there any to for me to seperate the merged s1.fq and s2.fq back into the 5 pairs of small fastq files?
Hope my description is clear. Thank you all!
I know that there have been many posts about readgroup in SAM/BAM format. But I am still confused.
Assume I have a sample named "Treated"sequenced with Illumina HiSeq platform. There are 5 pairs of the fastq files. I map each of the small fastq file using BWA and add read group when I merge the pairs of small bam files. For the first pair of bam files, can I just use "Treated_1" as its readgroup and "Treated_2" for the 2nd pair of bam files?
An opposite question: if I merge the fastq files for one end to get a file s1.fastq, then I merge the fastq files for the other end to get s2.fastq. Then I removed the original 5 pairs of original small fastq files. My question is, is there any to for me to seperate the merged s1.fq and s2.fq back into the 5 pairs of small fastq files?
Hope my description is clear. Thank you all!
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