Hi All,
Does anyone out there know of a good reference or two on the depth of coverage required to cover most transcripts and gauge expression levels in RNA-seq experiments? In particular, can anyone give me an idea of what percentage of sequences in their RNA-seq data sets are from what you would consider house-keeping genes? I have one reference in yeast from Wilhelm and Landry (2009) but I can't seem to find much else.
many thanks . . .
Does anyone out there know of a good reference or two on the depth of coverage required to cover most transcripts and gauge expression levels in RNA-seq experiments? In particular, can anyone give me an idea of what percentage of sequences in their RNA-seq data sets are from what you would consider house-keeping genes? I have one reference in yeast from Wilhelm and Landry (2009) but I can't seem to find much else.
many thanks . . .
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