How many cycles do you *really* get?

[jlove]

I'm interested in hearing how many cycles of sequencing people get out of various sequencing kits.

GA 36 cycle
HiSeq v2 50 cycle
HiSeq v2 200 cycle

I'd like to know how far your lab has been able to stretch the reagents, in a real world scenario (never mind what the "official" cycle number is).

[protist]

I'm interested in hearing how many cycles of sequencing people get out of various sequencing kits.

GA 36 cycle
HiSeq v2 50 cycle
HiSeq v2 200 cycle

I'd like to know how far your lab has been able to stretch the reagents, in a real world scenario (never mind what the "official" cycle number is).

We don't have a HiSeq but we routinely get 42 cycles from 36 cycle GA kit and can sometimes squeeze another cycle. For us the limiting solution is the Cleavage Reagent (CLM). IMX and SMX have another couple of cycles but you would be running on air for the CLM

[GW_OK]

A 200 cycle kit will actually get you 207 on the Hiseq, just enough for the index read.

[Baseless]

Also for the HiSeq Illumina officially includes enough reagents to do the indexing reads. So thats your window if you want to push it.

However, if you push it too much and have issues during fluidic checks/priming/flowcell loading, I would be a bit scared of the work after draining the instrument vs a few extra bases at the lower quality end of the read...