we want to sequece a self-cross line of maize, of which repeat sequnces accounts about 70%~80%. we plan to construct mult-insert size libraries, including 300bp, 500bp, 800bp,2000bp,5000bpand 8000bp and utilize 4 lanes to deliver about 50X data using solexa platform. so my question is
1) how to assign different insert size libraries to the 4 lanes?
2) does data of mate pair libraries produce a lot of redudant reads?
thanks a lot!
1) how to assign different insert size libraries to the 4 lanes?
2) does data of mate pair libraries produce a lot of redudant reads?
thanks a lot!
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