Hello,
I have GBS sequence file with 384 samples of two different parents. Now I want to separate individual sample based barcode sequence using Fastx barcode_splitter.pl program developed by Hannon Lab. The problem is this program require same length of barcodes. But I have different length of barcode. Is there any command line to make similar length of barcode based on barcode sequences and ApekI enzyme information?
Thanks
I have GBS sequence file with 384 samples of two different parents. Now I want to separate individual sample based barcode sequence using Fastx barcode_splitter.pl program developed by Hannon Lab. The problem is this program require same length of barcodes. But I have different length of barcode. Is there any command line to make similar length of barcode based on barcode sequences and ApekI enzyme information?
Thanks
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