Hi,
I am working on optimizing FFPE extraction for our next generation sequencing applications. I am currently interested in trying to repair the DNA, so that we have more dsDNA available for improved library construction yield and ultimately better quality sequencing.
I am looking into using the PreCR repair mix from NEB. I am also looking into using Restorase from Sigma-Aldrich. I was wondering if anyone has had any experience using these reagents for DNA repair? If not, does anyone have any advice on how to boost yields of dsDNA from FFPE material, either by repairing the DNA or by other methods? Any advice would be much appreciated, as obtaining adequate material from FFPE tissue can be tricky. Thanks!
I am working on optimizing FFPE extraction for our next generation sequencing applications. I am currently interested in trying to repair the DNA, so that we have more dsDNA available for improved library construction yield and ultimately better quality sequencing.
I am looking into using the PreCR repair mix from NEB. I am also looking into using Restorase from Sigma-Aldrich. I was wondering if anyone has had any experience using these reagents for DNA repair? If not, does anyone have any advice on how to boost yields of dsDNA from FFPE material, either by repairing the DNA or by other methods? Any advice would be much appreciated, as obtaining adequate material from FFPE tissue can be tricky. Thanks!
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