Hi! Ok, my sequencing reaction was all set up great, I cleaned with with "Centrisep" plates from Princeton Separations, and as a last step I fill any empty columns on my plate with Hi-Di formamide so as to not dry out my ABI 3730.
Question, I accidentally just went through and added Hi-Di Formamide to samples actually containing sequencing reaction that I needed. Will this in any way hurt or destroy the DNA, ruin the reaction, will I get OK results? Thank you so much in advance!
Question, I accidentally just went through and added Hi-Di Formamide to samples actually containing sequencing reaction that I needed. Will this in any way hurt or destroy the DNA, ruin the reaction, will I get OK results? Thank you so much in advance!
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