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  • #1

    Splice Junction for DNA

    Hi!!
    Can anyone introduce me a software to recognize splice junction on DNA sequence? I'm an undergraduate student and all these things are new for me..
    thanx.
    Tags: dna
  • #2
    Great question. ANNOVAR has limited ability to at least annotate exon-intron boundaries by defining X number of bases at the exon-intron boundary as "splicing".

    This is fairly inaccurate, but better than nothing.

    I do not know of a program that assesses splicing in next gen DNA sequencing data correctly. There seem to be quite a few for RNAseq analysis.

    There may be some older programs that may work for this purpose.
    Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
    Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
    Projects: U87MG whole genome sequence [Website] [Paper]

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    • #3
      Thank You very much.. I will try using this..

      Comment

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