degraded DNA

[dummyseq]

How can one assess if DNA sample is degraded before we start preparing the sample library for sequencing.

Nanodrop is not very accurate as per my knowledge, Quibit only gives starting input. If I run it on the Tapestation -which tape should I use and what should I expect -how can I tell the difference between a degraded and non degraded DNA sample.

Any input really appreciated.
Thanks

[AStretton]

Is it genomic DNA? If so you could just run a couple of ul on a gel, if you get a nice sharp band you are good to go, if it is a smear then likely its degraded.

[GW_OK]

Kappa has a genomic QC qPCR kit, assuming you're working with human DNA. It's kept us from wasting time on crappy samples.

[dummyseq]

what is an ideal amount one would load on a gel to observe DNA for degradation?

[AStretton]

We would load 2ul of stock gDNA