Hello, we're new to MiSeq and finding it difficult to relate the SAV information to our protocol to see what's going wrong.
Has anyone seen this sort of pattern (poor but increasing intensity)? We're seeing it regullarly when we use the Nextera XT protocol on long range PCR products but we're seeing much better data with our haloplex work and our home made capture kit so I'm at a loss to explain it.
Has anyone seen this sort of pattern (poor but increasing intensity)? We're seeing it regullarly when we use the Nextera XT protocol on long range PCR products but we're seeing much better data with our haloplex work and our home made capture kit so I'm at a loss to explain it.
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