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  • WGA techniques

    I am new in NGS. I am wondering if anybody knows the difference between 2 WGA methods:

    MALBAC of Sunney Xie

    vs

    Rubicon's PicoPlex

    I thought they are similar, but I could be wrong.

  • #2
    WGA, MALBAC and Rubicon

    Our blog post describes several single cell sequencing approaches. For more details check out their patents:

    Rubicon

    MALBAC

    Comment


    • #3
      They are all good when you have "few" cells, like 10 or more, but all suffer when you have a "single" cell. Mostly you will experience high allelic dropout and high noise in Copy number profiles. Currently, the method which is reported by multiple comparative articles to be the best performer for "single" cell WGA is Ampli1 http://www.siliconbiosystems.com/ampli1-wga-kit
      PLOS ONE | https://doi.org/10.1371/journal.pone.0193689 March 1, 2018
      PLOS ONE | DOI:10.1371/journal.pone.0171566 February 16, 2017

      Comment

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