Has anyone else noticed that the GTF files in the Illumina iGenomes collection don't conform to the GTF specification ? The attribute list must begin with gene_id then transcript_id whereas in the iGenomes GTFs it is gene_id then gene_name then either p_id or transcript_id. It would be nice if those extra attributes were at the end of the list so they worked with more programs.
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Yeah, well Illumina has always been known about going off on their own. In any case I question a program that can not parse an arbitrary field. If the order of the attribute list *must* be in a given order then the specification should have just made room for two more exact columns instead of relying on programs to do the parsing.
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Originally posted by fongchun View Post
Do you also get 0 FPKM when you use the GRCh37 from the Tophat site?
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Yes. I tried both the iGenome GRCh37 packages downloaded from Tophat and Cufflinks and both genes.gtf file gave me 0 FPKM for all genes when I just used cufflinks with the -G parameter.
It seems that maybe the current archive version (archive-2011-08-30-22-38-04) doesn't work? Anyone else have problems?
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It took me a week to drill back through my pipeline and work out these files were messing it up. In the end I got a fresh genome and GTF from ensembl, then used custom scripts to build all the various files req'd for picard, GATK etc. Its now working like a dream.
Lesson - if it looks too good to be true...@sidderb
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Originally posted by sidderb View PostIt took me a week to drill back through my pipeline and work out these files were messing it up. In the end I got a fresh genome and GTF from ensembl, then used custom scripts to build all the various files req'd for picard, GATK etc. Its now working like a dream.
Lesson - if it looks too good to be true...
Lesson - check to see if "chr" is appended to the chromosome number or not when dealing with datasets.
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