Hi
i have performed amplicon sequencing using gs junior and in the first run they were peaks around 120-150 bp.
so in the next run i want to reduce these peaks. i did 2x purification with the ampure beads(0.8: 1 ratio) but still there are slight or faint bands visible in agarose gel. How do i get rid of these bands completely?
if i am purifying it more further i am losing my ampliocns which are around 300bp.
Please help me.
Thanks
i have performed amplicon sequencing using gs junior and in the first run they were peaks around 120-150 bp.
so in the next run i want to reduce these peaks. i did 2x purification with the ampure beads(0.8: 1 ratio) but still there are slight or faint bands visible in agarose gel. How do i get rid of these bands completely?
if i am purifying it more further i am losing my ampliocns which are around 300bp.
Please help me.
Thanks
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