Hi,
I know this has been discussed previously (http://seqanswers.com/forums/showthr...ptome+assembly) , but I'm wondering if anyone can share their experiences on de novo transcriptome assembly with trimming and not trimming the 5' end due to the 'random' priming bias of illumina RNAseq reads. How much could this affect the assembly really?
Can anyone point to a paper that studied this?
I've always trimmed, but doing some recent updated searching, I'm almost seeing an equal proportion of both methods.
Thanks
I know this has been discussed previously (http://seqanswers.com/forums/showthr...ptome+assembly) , but I'm wondering if anyone can share their experiences on de novo transcriptome assembly with trimming and not trimming the 5' end due to the 'random' priming bias of illumina RNAseq reads. How much could this affect the assembly really?
Can anyone point to a paper that studied this?
I've always trimmed, but doing some recent updated searching, I'm almost seeing an equal proportion of both methods.
Thanks
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