exon capture mouse

[JRH]

Hi,

I am new here so I do not know whether this question was already posted somewhere:

I would like to capture the exome of mouse samples. Are there already commercial kits for that? I can only find human exome capture methods.
With the Agilent Sureselect system we may make our own, but that is likely very expensive...

Best wishes,
Jurgen

[josdegraaf]

Hi Jurgen,

We are about to recieve a pre-release mouse whole exome kit from Agilent by the end of the week.

[krobison]

Also, with Agilent you can pretty easily design capture reagents for any of their supported genomes -- though it's 4-6 weeks to get your reagents.

I think you can do unsupported genomes too, but that's outside my current scope.

[acpatel]

kit availability? cost?

Jurgen,

Any word on how the kit performs? Cost? Availability/

Thanks,
Anand

[SureSelectGuru]

Agilent Technologies Introduces Mouse Exome Target Enrichment Kit

http://www.agilent.com/about/newsroom/presrel/2011/19jan-ca11004.html

[Robby]

Hi,
has someone already experience with the Agilent SureSelect mouse whole exome kit? Does it work well? How many reads map to the bait covered regions? How is the coverage distribution?

Are there any other All Exon kits for mouse available (i.e. from Nimblegen, Illumina,...)?

Thanks,
Robby