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Old 08-18-2010, 08:09 AM   #1
zheng
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Default Human DNA with whole genome sequenced?

Hi, I'm wondering if there's any publicly available human DNA with whole genome sequenced and released? I'm doing some capture sequencing, I guess I need some standard samples to see if I can discover all the variants in my target region, the false positive and false negative rate.
Thanks!
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Old 08-18-2010, 01:23 PM   #2
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The HapMap DNAs are available. They've been genotyped for 1.5 million SNPs
http://ccr.coriell.org/sections/coll....aspx?PgId=266
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Old 08-18-2010, 01:25 PM   #3
james hadfield
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email Craig Venter or James Watson for some?
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Old 08-18-2010, 01:49 PM   #4
zheng
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Thanks for the replies. Most of the hapmap samples were only genotyped for the common SNPs, not sequenced. It may be easier to sequence my own whole genome than to get Venter or Watson's DNA.

Actually I think I found the answer, Illumina sequenced the whole genome of an African sample and released the data, the sample is also a Hapmap sample that can be bought from Coriell.
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Old 08-18-2010, 05:09 PM   #5
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Quote:
Originally Posted by zheng View Post
Thanks for the replies. Most of the hapmap samples were only genotyped for the common SNPs, not sequenced. It may be easier to sequence my own whole genome than to get Venter or Watson's DNA.

Actually I think I found the answer, Illumina sequenced the whole genome of an African sample and released the data, the sample is also a Hapmap sample that can be bought from Coriell.
U87MG was sequenced on SOLiD, and a very common cell line.
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Old 08-20-2010, 06:41 PM   #6
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Craig Venter's DNA is available from the Coriell Institute.
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Old 09-08-2010, 03:52 AM   #7
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What about any of the high coverage 1000 genomes samples, NA12878 or NA19240 for example
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Old 09-08-2010, 11:48 AM   #8
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I used to post it in another thread:

ftp://ftp.sanger.ac.uk/pub/rd/humanSequences/

These data are processed in the same way.
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