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  • the latest pipeline PL 1.4

    I've been looking around at different sequencing options and it seems that most Illumina people are running older pipelines and the amount of sequence generated (~500 MB/lane) is only useful for whole genome sequencing of microbes with smaller genomes and Chip-seq experiments.

    Anyone know of places running newer pipelines that can generate the amount of sequence per lane to make worm and fly genome sequencing more palatable? Are these places willing to take orders from outside?

    looking around it seems that even though pipeline 1.4 came out this year a lot of places are still running 0.3 (not that that's bad) which isn't optimal for my purposes.

    Thanks for your suggestions.

  • #2
    Ya it's surprising to me too considering how much better the data from 1.4 is (yield and quality). As for your question:

    I know the major genome centers (Sanger, Wash U, Bayer, BGI) are all running the latest or close to it. Illumina's service center in Hayward, CA are also running 1.4.

    Brad

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