Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • solid2fastq paired-end

    Hi SOLiD user,
    i have to align SOLiD reads to human genome. They are from a paired-end experiment, so I have a csfasta and a quality file for F3 and for F5-P2.

    For example I have these files:
    solid_data_F3.csfasta
    solid_data_F5-P2.csfasta
    solid_data_F3_QV.qual
    solid_data_F5-P2_QV.qual

    To obtain a fastq file, is correct the following command?
    > solid2fastq.pl solid_data_ output

    In this way, i obtain only one file to align to genome. Is it correct?
    thanx to everybody
    ME

  • #2
    Originally posted by m_elena_bioinfo View Post
    Hi SOLiD user,
    i have to align SOLiD reads to human genome. They are from a paired-end experiment, so I have a csfasta and a quality file for F3 and for F5-P2.

    For example I have these files:
    solid_data_F3.csfasta
    solid_data_F5-P2.csfasta
    solid_data_F3_QV.qual
    solid_data_F5-P2_QV.qual

    To obtain a fastq file, is correct the following command?
    > solid2fastq.pl solid_data_ output

    In this way, i obtain only one file to align to genome. Is it correct?
    thanx to everybody
    ME
    What package is the "solid2fastq.pl" script from?

    Comment


    • #3
      i'm using solid2fastq.pl in bwa0.5.7 package

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      40 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      41 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      36 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X