Tweet
Hello! I an preparing the samples and designing the analysis scheme for RNA-seq. I want to use two groups of samples to do differential analysis, do all the samples in a group need to be biological replicates? Can it be technical replicates?
-
-
Of course you need biological replicates! What you're going to be testing, for each gene, is whether the difference between groups is greater than the difference within groups. You can't do that if you don't know the differences within the groups. See doi:10.1093/bioinformatics/btt688 for some numbers.
Technical replicates were standard with microarrays because of the technical noise, but that's not really an issue for RNA-seq so they're not usually done. -
Hi, Jwfoley, thanks a lot.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment