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  • High coverage, suspecting a problem...

    We've sequenced an exome (SureSelect) with our HiSeq .

    I've aligned the reads with bwa & marked the duplicates. the qualities of the reads seem to be OK.

    I'm puzzled: I've got a very high coverage in the target regions (mean: ~150/200) and I'm looking for a way to explain it.

    For a sample the size of the FASTQs is 10Gb.
    Whereas for a previous sequencing of the same sample the size of the FASTQs was 4.G (The size of the illumina folder was ~150Gb with both folders).

    What would you suggest to explore the reason of this high coverage ?

    I've extracted my FASTQs using the following command (with the default options):

    Code:
    ${CASAVADIR}/bin/configureBclToFastq.pl \
    	--input-dir ${BCLDIR}/Data/Intensities/BaseCalls \
    	--output-dir ${FASTQDIR} \
    	--sample-sheet ${BCLDIR}/SureSelect2.csv \
    	--force
    should have I used some other options ?


    Thanks,

    Pierre

  • #2
    I am not expert in bioinformatics before going further I suggest you to compare the cluster numbers and millions of reads of both flow cells. If they both are same then I guess you may have to look at the bioinformatics steps.

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