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RNA-Seq: Length Bias Correction for RNA-seq Data in Gene Set Analyses. Newsbot! Literature Watch 0 01-22-2011 02:02 AM

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Old 02-07-2018, 11:16 AM   #1
l_zembek
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Location: Cambridge, MA

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Default 5' RNA-Seq Bias?

What are some possible reasons for 5->3' bias in RNA seq data? Libraries were generated using Illumina's TruSeq Stranded mRNA protocol, with pretty good quality RNA input. If anything, with a polyA selection method, I would think there'd be 3' bias.

Thanks,
Lisa

Last edited by l_zembek; 02-08-2018 at 09:19 AM.
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