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  • RNASeq & Whole Genome(DNA) Seq Analysis Training in Mumbai, India

    WHAT: 2-day Technical Training in NGS Data Analysis | Whole Genome (DNA) Sequencing & RNASeq in Mumbai, India

    WHEN: 6-7 March 2020

    It will be an extensive 2-day training program for which participants have to bring their own laptop having Windows/Mac/Linux so the participants can use that for running the processes on their own computer systems- HANDS-ON with complete learning of Next Generation Sequencing Data Analysis. The program will be of great help to students/ researchers/ faculty/ scientists in learning the analysis of NGS DNA & RNA Data.

    Event Website

    PRACTICAL APPLICATION
    In the workshop, we will perform 2 experiments to analyze the example DNASeq & RNASeq Data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.

    SCHEDULE | DAY 1 EXPERIMENT 1
    In this lesson, we will focus on the SNPs and Variant Analysis (VC) of the DNASeq data and cover:
    • Deal with the raw data for quality evaluation and filtering
    • Map reads to the reference genomes, call SNPs and predict their phenotype consequence
    • Alignment to a reference genome, SNPs and In-dels Calling of next generation DNA data


    SCHEDULE | DAY 2
    EXPERIMENT 2 In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
    • Upload Data
    • Convert File Format
    • Align Reads To Reference Genome
    • View The Mapped Reads In JBrowse
    • Count Reads Per Gene
    • Test For Differential Expression
    • Differential Gene Expression
    • MDS Plot
    • MA Plot
    • Volcano Plot
    • Parallel Coordinates and Heatmap
    • Investigate Differentially-Expressed Genes
    • Chord Diagram

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