|Thread||Thread Starter||Forum||Replies||Last Post|
|PacBio Library Prep workshop and PacBio SMART-Portal bootcamp - UC Davis - April 2015||DNATECH||Events / Conferences||1||04-02-2015 08:33 AM|
|RNA -> cDNA libraries on PacBio||snf||Pacific Biosciences||3||01-23-2015 09:26 AM|
|Good way to make cDNA libraries for one-embryo RNA-seq||Yui||General||0||12-19-2014 04:39 AM|
|Had a question, Need some advice||break4minutes||Introductions||2||06-07-2012 10:15 AM|
|Sequencing advice||nkaushik||Bioinformatics||2||05-31-2012 01:23 PM|
|12-19-2016, 10:27 PM||#1|
Location: Haifa, Israe;
Join Date: Feb 2013
Advice on getting good PacBio libraries
We are having issues getting good quality PacBio libraries from Bacteria and microalgae. Specifically, we got low yield (76 MB) and low length (5,800 bp) from several libraries run on separate occasions. Other samples run in parallel had good stats, so the problem was not in the instrument and likely not in the library prep at our supplier.
The DNA samples looked OK on a gel before and after shearing, but the yields were lower than expected after size selection from library prep.
Has anyone experienced difficulty in getting the appropriate DNA for PacBio to work well? We could only get one out of four libraries to sequence well... Any advice would be useful.
One point to consider is that we sent the samples using GenTegra tubes rather than on dry ice. Does anyone have experience with these tubes for PacBio?
|dna, gentegra, pacbio, quality|