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Old 06-17-2011, 02:58 AM   #1
nikiwilson
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Location: cambridge

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Default primer/adapter sequences

Hi,
can anyone please help??? I have been using the Illumina ChIP-Seq amplification kit for a while now and would like to move to the NEB kit. The NEB kit doesn't contain the primers/adapters and they suggested looking on this forum for the sequences, can anyone help???? Where would you normally order them from?? Do they need to be HPLC purified????
Many thanks
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Old 06-21-2011, 01:42 PM   #2
liupf56
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See the attached file. Use PE adapters and PE PCR primers on page 3. You can order them from IDT and choose PAGE purification.
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File Type: pdf PM protocol _genomic DNA_1.0.pdf (237.7 KB, 601 views)
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Old 06-21-2011, 02:36 PM   #3
NextGenSeq
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Note that for best results the bond between the last 2 bases on the 3' end must be a phosphothioate bond. Most oligo companies can do this. If you don't modify the oligo you will get adapter artifacts.
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