SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
supplier for Illumina reagents M&Morenos Illumina/Solexa 3 02-03-2014 03:49 AM
Illumina nextra sequencing Bgansw Bioinformatics 2 11-05-2013 07:08 AM
Post-expiry Illumina reagents ilmns79 Illumina/Solexa 3 09-27-2013 12:10 PM
Bad Illumina Reagents aleferna Illumina/Solexa 2 05-13-2011 06:56 AM
What happens when Illumina sequencing reagents run out? picabo Illumina/Solexa 2 01-17-2011 07:46 AM

Reply
 
Thread Tools
Old 04-08-2015, 06:37 AM   #1
crsimao
Junior Member
 
Location: new york

Join Date: Apr 2015
Posts: 1
Default Illumina Nextra prep without using Illumina reagents

Hi everyone,
I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.
crsimao is offline   Reply With Quote
Old 04-08-2015, 11:21 AM   #2
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,194
Default

Quote:
Originally Posted by crsimao View Post
Hi everyone,
I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.
Realistically? No. I don't think so.

--
Phillip
pmiguel is offline   Reply With Quote
Old 04-08-2015, 10:30 PM   #3
bunce
Member
 
Location: Perth

Join Date: Sep 2012
Posts: 55
Default

By 'prep' do you mean library prep? - if so, then yes, there are lots of ways of building libraries that don't require Illumina kits. There are home brew methods and other suppliers (e.g. the NEB next range). If you dig a little deeper online you will find some protocols (e.g we use; http://www.ncbi.nlm.nih.gov/pubmed/21431776) - the only advice I would give is to be certain of your adapter sequences as there are a number of historical tweaks that have been made.
bunce is offline   Reply With Quote
Old 04-08-2015, 10:42 PM   #4
nucacidhunter
Senior Member
 
Location: Iran

Join Date: Jan 2013
Posts: 950
Default

Look at this paper for home brew Nextera library prep:http://genome.cshlp.org/content/24/12/2033.full It is only worth if you are going to prepare lots of libraries and have facilities for protein expression and purification.
nucacidhunter is offline   Reply With Quote
Old 04-14-2015, 10:29 AM   #5
Svetlana
Junior Member
 
Location: madison

Join Date: Dec 2011
Posts: 1
Default

Quote:
Originally Posted by crsimao View Post
Hi everyone,
I was hoping some here could help me, i was wondering if there was anyway to sequencing on a miseq machine without having to use Illumina prep reagents. I know the cartridge that goes into the machine needs to be from illumina but i wanted to see if there is a way to do the prep without having to buy there expensive reagents.
Also, you can visit http://lucigen.com/NextGen-Seq/ to find protocols and kits to make fragment and mate pair libraries for sequencing on Illumina platform.
Svetlana is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:33 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO